BIOREADER®-7000-V

Bioreader®-7000-V
Non-fluoro applications in 384 well –6 Well plates and Petridishes.

BIOREADER®-7000-V
Bioreader®-7000-V
Non-fluoro applications in 384 well –6 Well plates and Petridishes.

The BIOREADER®-7000-V is equipped with a fully automatic fixed lens changer.

Wells between 384 and 6 well as well as Petridishes 35 mm - 1000 mm may be visualized full field of view.

Providing a resolution for  Foci 100-2000 µ or Plaques 100-5000 µ, cell clones 50 - 2000 µ and more.

Modell variants

Alpha for 96 + 24/12 wells.

Beta for 384/96, 48/24/12 and 6 wells.

Gamma for 384/96, 48/24/12 und 6 well multiwell plates and Petri dishes.

 

Non-Fluoro-substrats and staining

This BIOREADER®-7000 is  specially designed for visible CV or  immunochemical substrates.

The panted dual telecentric illumination suppresses reflection the sidewall of multifilterplates.

Elispot with all available substrates like AEC,Silver,TMB or BCIP/NBT

Enzyme conjugates like Alkaline Phosphatase (ALP) or Horseradish Peroxidase (HRP) 

Antiviral vaccine development by use of the Bioreader®

This Bioreader ® supports testing and development of  antiviral vaccines.

Example#1: The automatic analyses of   Plaque reductions assay.

This assay applies diluted doses of a -possibly- antiviral drug into microwell plates. The Bioreader automatically observes its capability to suppress the formation of plaques(-holes-) in the bacterial lawn.

The ‘Plaque reductions neutralization test’ (PRNT) is a variation of this test. It is an attempt to find an antibody that neutralizes a specific virus. This is a very valuable tool in antiviral vaccine research.

Examplel#2: The automatically evaluation of the Yield reduction assay

In several steps of operation cell are infected in presence of variable doses of a possibly antiviral drug substance.

Thereafter the virus titer is determined in the liquid by use of the plaque or TCID50 assay with of  the Bioreader®.

The titer is calculated as the quotient of the positive cells/all cells, taking in account the virus dilution factor and the volume of the inoculum.

Features
  • Patented dual telecentric illumination
  • ’EazyReader® software generation 20’
  • Front loader with automatic door
  • Fold lens changer with high definition fix focus lenses
  • Automated focus, illumination, lens changer are measure protocol controlled and locked
  • Standard 5 MPixel camera (up to 42 MPixel available)
  • Optional 21 cfr part 11 software module (GAMP IQ/OQ/PQ/DQ/FAT/SAT)
  • DELL PC with 3 years NBD on-site service and warranty (Office/Windows 10)
  • Loudspeakers
  • For easy and powerful usage, incl. Q.M. package
  • Excel/Word/PowerPoint and LIMS export capabilities
  • Interactive content specific video training program
  • ‘Clinical routine’ and ‘research’ user interface
  • For ‘phantom spot suppression’, better spot separation and cytokine quantification
Software

biocreader_7000_v_software.png

Eazyreader® software

  • Innovative Eazyreader® software combines ‘easy of use’ and versatility and flexibility
  • Optimized applications for commonly used operations like ‘routine scan’
  • QC presents only the icons you requires for this job
  • Scan, analyze and overlay live time creates up to 7 images for each well simultaneously during te scan already
  • ‘Profiling’ help to create ‘user independent’ measure protocols for Elispot beginners and references fro experts
  • Verified in collaborative studies
  • Export options including reports with all scalable images and results in one file
  • Customer specific report templates
  • Video clips and content specific help files
  • Qualified installation and training on side with each Bioreader®
  • On side support and internet remote support
  • ‘Classified’ measure protocols ‘history’ tracking and comparison tools
  • User specific projects, designs, protocols and studies prevent from mixup
  • More accurate ‘cytokine quantification’ based on the patented ‘photometric’ dual illumination system
  • CE EMV certificate
  • 21 cfr part 11 module
  • Full automation optional with ‘feeding system’

 

Dimension

width
45 cm
height
60 cm
depth
35 cm

Weight

weight
26 kg
The BIOREADER®-7000-V can also use with the follow applications:
Plaque assay.Very bright plaques in 24 well plate Icon

Plaque assay.Very bright plaques in 24 well plate

Plaque assay with very bright plaques in 24 well plate

Cells were infected and covered with an overlay. Surviving cells stained with CV. Cells killed by the virus.

Foci assay: total well view Icon

Foci assay: total well view

Foci assay: total well view

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed." [https://en.wikipedia.org/wiki/Virus_quantification]

Elispot in 384 multifilterplates Icon

Elispot in 384 multifilterplates

Elispot in 384 multifilterplates & Elispot single encymatic

Small volume, high throughput encymatic Elispot

"The enzyme-linked immune absorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell." [https://en.wikipedia.org/wiki/ELISpot]

Elispot single enzymatic Icon

Elispot single enzymatic

Elispot single encymatic

Blue/red/green/silver substrat Elispot. Detection antibody, enzyme-conjugate and precipitating substrate.

Why to use Elispot

•The cytokine Elispot assay is designed to enumerate cytokine secreting cells in single cell suspensions of lymphoid tissue, CNS tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMC).

•The assay has the advantage of detecting only activated/memory T cells and the cytokine release can be detected at the single cell level, allowing direct determination of T cell frequencies.

•The assay has the advantage of detecting only activated/memory T cells and the cytokine release can be detected at the single cell level, allowing direct determination of T cell frequencies.

The ELISPOT assay is an effective tool to enumerate antigen-specific T cells in the circulation of immunized humans and animals at much lower frequencies than possible with other currently available methods

The ELISPOT assay has proven to be a sensitive and unique system to follow disease progression in human individuals or animals. Several studies have indicated that alterations in the frequency of cytokine pc in different compartments of the body adequately reflect changes in immune function

The ELISPOT assay may be used to determine effects of drugs, chemicals or other compounds on cytokine secretion in vitro, thereby providing data on their putative modulatory effects on immune function in vivo

Dual Elispot enzymatic 1-2 colors Icon

Dual Elispot enzymatic 1-2 colors

Dual Elispot enzymatic

1-2 colors

Blue/red substrate and mixed color violet. Same as sample #18-11 but 2 colors.

non stained cells Icon

non stained cells

Non stained cells may be counted in a kind of phase contrast mode.

Alveolar epithelial cells (AEC) Icon

Alveolar epithelial cells (AEC)

Alveolar epithelial cells (AEC)

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis." [https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epitheliumis a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cellsare important in maintaining the integrity of alveolar epitheliumand normal lung function.

MCMV: mouse cytomegalovirus, unstained Icon

MCMV: mouse cytomegalovirus, unstained

MCMV: mouse cytomegalovirus, unstained

HCMV Human cytomegalovirus, unstained Icon

HCMV Human cytomegalovirus, unstained

HCMV human cytomegalovirus, unstained

"The glycoprotein gO (UL74) of human cytomegalovirus (HCMV) forms a complex with gH/gL. Virus mutants with a deletion of gO show a defect in secondary envelopment with the consequence that virus spread is restricted to a cell-associated pathway." [https://www.ncbi.nlm.nih.gov/pubmed/20181688]

NK92 cells natural killer cells (human cell line) same samples microwell plate. Icon

NK92 cells natural killer cells (human cell line) same samples microwell plate.

NK92 cells natural killer cells (human cell line) same samples microwellplate

"Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. Typically, immune cells detect major histocompatibility complex (MHC) presented on infected cell surfaces, triggering cytokine release, causing lysis or apoptosis." [https://en.wikipedia.org/wiki/Natural_killer_cell]

Foci assay: Very large Foci Icon

Foci assay: Very large Foci

Foci assay: very large Foci

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed." [https://en.wikipedia.org/wiki/Virus_quantification]

Plaque assay extremely large spots Icon

Plaque assay extremely large spots

Plaque assay with extremely large spots

"The TCID50 (Median Tissue Culture Infectious Dose) is one of the methods used when verifying viral titer. TCID50 signifies the concentration at which 50% of the cells are infected when a test tube or well plate upon which cells have been cultured is inoculated with a diluted solution of viral fluid." [https://www.zeomic.co.jp/en/glossary/virus/71]

CV Plaque assay.Example with large and fuzzy plaques Icon

CV Plaque assay.Example with large and fuzzy plaques

CV Plaque assay; example with large and fuzzy plaques

Cells were infected and covered with an overlay. Surviving cells stained with CV. Cells killed by the virus.

TCID50 viral assay Icon

TCID50 viral assay

Various media/strain combinations on petridishes Icon

Various media/strain combinations on petridishes

Various media/strain combinations on petridishes

Dual Transgene assay Icon

Dual Transgene assay

Dual Transgene assay

A segment of DNA introduced to some other organism. See [https://en.wikipedia.org/wiki/Transgene]

Various colonies in 6 well plates and Petridishes 35 mm - 100 mm Icon

Various colonies in 6 well plates and Petridishes 35 mm - 100 mm

Various colonies in 6 well plates

Organoids Icon

Organoids

Organoids

"An organoid is a miniaturized and simplified version of an organ produced in vitro in three dimensions that shows realistic micro-anatomy. The technique for growing organoids has rapidly improved since the early 2010s." [https://en.wikipedia.org/wiki/Organoid]

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 488 labelled secondary antibodies Icon

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 488 labelled secondary antibodies

Detection of Marek's disease virus

Plaques after staining with specific anti-MDV antibodies and Alexa 488 labelled secondary antibodies

"Marek's disease is a highly contagious viral neoplastic disease in chickens. The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes. Viruses related to MDV appear to be benign and can be used as vaccine strains to prevent Marek's disease." [https://en.wikipedia.org/wiki/Marek's_disease]

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 568 labelled secondary antibodies Icon

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 568 labelled secondary antibodies

Detection of Marek's disease virus but Alexa 568 labelled

"Marek's disease is a highly contagious viral neoplastic disease in chickens. The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes. Viruses related to MDV appear to be benign and can be used as vaccine strains to prevent Marek's disease. " [https://en.wikipedia.org/wiki/Marek's_disease]

Neutralization assay Accepted Plaques are surrounded Icon

Neutralization assay Accepted Plaques are surrounded

Neutralization assay

Accepted Plaques are surrounded

"The plaque reduction neutralization test is used to quantify the titer of neutralizing antibody for a virus. The serum sample or solution of antibody to be tested is diluted and mixed with a viral suspension." [https://en.wikipedia.org/wiki/Plaque_reduction_neutralization_test]

Neutralization assay Icon

Neutralization assay

Neutralization assay

"The plaque reduction neutralization test is used to quantify the titer of neutralizing antibody for a virus. The serum sample or solution of antibody to be tested is diluted and mixed with a viral suspension." [https://en.wikipedia.org/wiki/Plaque_reduction_neutralization_test]

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