BIOREADER® 7000 -F

Fluoro Elispot/Plaque + Enzymatic Readers for 96 wells with innovative power LED fluoro excitation.

BIOREADER® 7000 -F
Fluoro Elispot/Plaque + Enzymatic Readers for 96 wells with innovative power LED fluoro excitation.

This BIOREADER® 7000 -F allows you to evaluate verified enzymatic Elispot/Plaque or Fluorospot counting.

They are equipped with a long-lasting LED fluorescent light source. This allows you to evaluate verified enzymatic Elispot or Fluorospot counting.

This BIOREADER® 7000 -F applies up to 10 standard Olympus® flouro filter cubes automatically.

Model types:

                                    Filter cubes installiert        Max. number of filter cubes

Alpha                                           2                                               6

Beta                                             3                                               6

Gamma                                       4                                              10

Delta                                            4                                              10

The Delta model has an additonal UV LED light source installed for DAPI filters.

 

Flexibility

The Bioreader® hardware may be upgraded on site. Brickstone principle.

Independence

Bioreader® operates independent form any assays or kit manucafurer, so you may choose among all assay/kit manufactures.

 

Features
  • 6- or 10-times fluoro filter changer: For Standard Olympus® filter cubes on all models.
  • New high sensitive but low noise camera technology with optimal lens glasses for high contrasted images.
  • Filter upgrade options: From model Alpha up to Delta (max 10 filter cubes).
  • 3D spot-volume cytokine quantification: Provides more sensitive stimulation response results.
  • Bioreader® 7000 -F is an affordable model for fluoro and enzymatic Elispot and Plaque/FOCI counting in 384/96 well plates.
  • Dual tele-centric illumination.
  • Counting significantly faster and more precise regarding positioning.
  • Front loader, automatic door.
  • Loud speaker and interactive training program.
  • Excel/Word/Power Point and LIMS export capabilities.
  • Suiteable for reserarch purpose.
  • Camera upgrade to 6 - 41 MPixel available.
  • Optional full automation with plate 'feeding system'.
  • DQ/IQ/OQ/PQ documentation possible
  • 12 month warranty.
Software

biocreader_7000_f_software.png

Eazyreader® software

  • Innovative EazyReader® software combines ‘easy of use’ and versatility and flexibility.
  • Scan, analyze and overlay live time.
  • Creates up to 7 images for each well simultaneously during the scan.
  • ‘Profiling’ app helps to create user independent measure protocols for Elispot beginners and references for experts.
  • Verified in collaborative studies.
  • Export options and reports with all scalable images and results even in one file.
  • Customer specific export and report templates.
  • Video clips and content specific help files.
  • Qualified installation and training with each Bioreader® model.
  • On-site or internet remote services and support.
  • ‘Classified’ measure protocols ‘history’ tracking and comparison tools.
  • User specific plates, Studies/projects, plate layouts/designs and measure protocols prevent from mix-up.
  • More accurate ‘cytokine quantification’ based on the patented ‘photometric’ dual illumination system.
  • Optional software features:
  • 'Routine’ software package: Ooptimized applications for commonly used operations, only presents the icons you require for the specific job, quickly read a plate and quickly review, Q.C. and release the results.
  • 21 CFR part 11 based software module available.

Dimension

width
60 cm
height
60 cm
depth
45 cm

Weight

weight
35 kg
The BIOREADER® 7000 -F can also use with the follow applications:
Fluorospot 1-4 fluorophores Icon

Fluorospot 1-4 fluorophores

Multi Fluoro Color Reader,

Best for Elispot and FluoroSpot.

 

Applies ‘local neighborhood processing’ in order to compensate for uneven background on fluoro plates. This way the spot volume is quantified, independent of the local background.

 

Why use Fluorospot?

Enzymatic Elispot labeling
• allows to label 1-2 cytokines per cell simultanously.
• If you are interested to see ‘double secreting’ cells the stain of two cells in neighborhood may overlap. So, It is not clear if two cells are overlapping or one cells is really secreting two different cytokines ( see below left)

 

‘Fluorospot’ labeling
Allows to label  -a theoretical unlimited number different-  cytokines. Because excitation for each cytokine is applied -one after the other-, decisions for double secreting cells is clearly.
One Image is acquired for each cytokine!

Currently Fluorospot assays with 1-4 Fluorophores are on the market.

Fluorospot offer's significant advantages over colorimetric formats, particularly in the areas of multiplexing and automated spot detection.

Moreover, as spot development is not enzymatic, signal intensity is directly proportional to the amount of analyte within.

 

Elispot in 384 microfilter plates Icon

Elispot in 384 microfilter plates

Small volume, higher throughput.

Elispot - enzymatic single color Icon

Elispot - enzymatic single color

Elispot single encymatic

Blue, red, green or silver substrat Elispot.

Detection antibody, enzyme-conjugate and precipitating substrate.

"The enzyme-linked immune absorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell."

[https://en.wikipedia.org/wiki/ELISpot]

Why to use Elispot

•The cytokine Elispot assay is designed to enumerate cytokine secreting cells in single cell suspensions of lymphoid tissue, CNS tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMC).

•The assay has the advantage of detecting only activated/memory T cells and the cytokine release can be detected at the single cell level, allowing direct determination of T cell frequencies.

•The assay has the advantage of detecting only activated/memory T cells and the cytokine release can be detected at the single cell level, allowing direct determination of T cell frequencies.

The ELISPOT assay is an effective tool to enumerate antigen-specific T cells in the circulation of immunized humans and animals at much lower frequencies than possible with other currently available methods

The ELISPOT assay has proven to be a sensitive and unique system to follow disease progression in human individuals or animals. Several studies have indicated that alterations in the frequency of cytokine pc in different compartments of the body adequately reflect changes in immune function

The ELISPOT assay may be used to determine effects of drugs, chemicals or other compounds on cytokine secretion in vitro, thereby providing data on their putative modulatory effects on immune function in vivo

Elispot - enzymatic dual color Icon

Elispot - enzymatic dual color

Blue and red Elispot substrate (mixed color violet)

Dual cytokine secretion.

RFP-expressed virus infected Icon

RFP-expressed virus infected

RFP-expressed virus infected

"Using DNA recombinant technology, scientists combine the RFP gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell." [https://embryo.asu.edu/pages/green-fluorescent-protein but with RFP instead of GFP]

non stained cells Icon

non stained cells

Non stained cells may be counted in a kind of phase contrast mode.

Alveolar epithelial cells (AEC) Icon

Alveolar epithelial cells (AEC)

Alveolar epithelial cells (AEC)

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis." [https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epitheliumis a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cellsare important in maintaining the integrity of alveolar epitheliumand normal lung function.

MCMV (Murine Cytomegalovirus) greenfluoro remission Icon

MCMV (Murine Cytomegalovirus) greenfluoro remission

MCMV (Murine Cytomegalovirus)

green fluoro remission

"Cytomegalovirus (CMV) (from the Greek cyto-, "cell," and megalo-, "large") is a genus of viruses in the order Herpesvirales." [https://en.wikipedia.org/wiki/Cytomegalovirus]

MCMV (Murine Cytomegalovirus) red fluoro remission. Icon

MCMV (Murine Cytomegalovirus) red fluoro remission.

MCMV (Murine Cytomegalovirus)

red fluoro remission

MCMV: mouse cytomegalovirus, unstained Icon

MCMV: mouse cytomegalovirus, unstained

MCMV: mouse cytomegalovirus, unstained

HCMV Human cytomegalovirus, unstained Icon

HCMV Human cytomegalovirus, unstained

HCMV human cytomegalovirus, unstained

"The glycoprotein gO (UL74) of human cytomegalovirus (HCMV) forms a complex with gH/gL. Virus mutants with a deletion of gO show a defect in secondary envelopment with the consequence that virus spread is restricted to a cell-associated pathway." [https://www.ncbi.nlm.nih.gov/pubmed/20181688]

MCMV mutants got generated that express with tdTomato, eGFP or mCherry Icon

MCMV mutants got generated that express with tdTomato, eGFP or mCherry

MCMV mutants got generated that express with tdTomato, eGFP or mCherry

MCMV mutants, tdTomato, Cherry, eGFP fibroplasts

GFP immunofluorescence on HEK 293 cells Icon

GFP immunofluorescence on HEK 293 cells

GFP immunofluorescence on HEK 293 cells

"Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cellsgrown in tissue culture." [https://en.wikipedia.org/wiki/HEK_293_cells]

NK92 cells natural killer cells (human cell line) expressing green fluorescent protein (gfp) Icon

NK92 cells natural killer cells (human cell line) expressing green fluorescent protein (gfp)

NK92 cells natural killer cells (human cell line) expressing green fluorescent protein (gfp)

"Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. Typically, immune cells detect major histocompatibility complex (MHC) presented on infected cell surfaces, triggering cytokine release, causing lysis or apoptosis." [https://en.wikipedia.org/wiki/Natural_killer_cell]

NK92 cells natural killer cells (human cell line) same samples microwell plate. Icon

NK92 cells natural killer cells (human cell line) same samples microwell plate.

NK92 cells natural killer cells (human cell line) same samples microwellplate

"Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. Typically, immune cells detect major histocompatibility complex (MHC) presented on infected cell surfaces, triggering cytokine release, causing lysis or apoptosis." [https://en.wikipedia.org/wiki/Natural_killer_cell]

Mouse spleen cells mostly lymphocytes stained with FITC-conjugated anti mouse cd45 stain Icon

Mouse spleen cells mostly lymphocytes stained with FITC-conjugated anti mouse cd45 stain

Mouse spleen cells mostly lymphocytes stained with FITC-conjugated anti mouse CD45 stain

"The spleen is an organ found in virtually all vertebrates. Similar in structure to a large lymph node, it acts primarily as a blood filter. A study published in 2009 using mice found that the red pulp of the spleen forms a reservoir that contains over half of the body's monocytes." [https://en.wikipedia.org/wiki/Spleen]

Foci assay: total well view Icon

Foci assay: total well view

Foci assay: total well view

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed." [https://en.wikipedia.org/wiki/Virus_quantification]

Foci assay: Very large Foci Icon

Foci assay: Very large Foci

Foci assay: very large Foci

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed." [https://en.wikipedia.org/wiki/Virus_quantification]

GFP/DAPI-expressed virus infected Icon

GFP/DAPI-expressed virus infected

GFP/DAPI-expressed virus infected

"Using DNA recombinant technology, scientists combine the GFP gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell." [https://embryo.asu.edu/pages/green-fluorescent-protein]

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